Almanza G, Rodvold JJ, Tsui B, Jepsen K, Carter H, Zanetti M (2018) Extracellular vesicles produced in B cells deliver tumor suppressor miR335 to breast cancer cells disrupting oncogenic programming in vitro and in vivo. Nature Scientific Reports 8:17581. DOI:10.1038/s41598-018-35968-2.
Researchers at the University of California, San Diego leveraged a new technical approach using extracellular vesicles (EVs) laden with a tumor suppressor miRNA (miR-335) produced in B cells by plasmid DNA induction (iEVs). Their study demonstrated that iEVs are programmable to contain miR-355 cargo, deliver and restore it in LM2 cells, modulate target mRNA expression in vitro and in vivo, and greatly reduce the growth of orthotopic LM2 tumors in immune-deficient mice.
The study indicates that iEVs-335 could provide a new form of therapeutic intervention in cancers in which genomic interrogation documents a decrease of tumor suppressor miR-335 and/or an increase of SOX4, a transcription factor involved in embryonic development and cell fate determination.
RNAGEM was used for RNA extraction from transfected or untransfected J558L cells as well as for extracellular vesicles (EV) extraction.
This study is also referenced in MicroGEM's RNAGEM Technical Overview.