Product Document
Biopharma Resources
Increase process productivity, reduce workflow costs, minimize risk, and bring products to market faster with prepGEM® Universal and RNAGEM™
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RNAGEM
Temperature-driven, single-tube extractions of total RNA from mammalian cells, tissues, insects, bacteria and virus
This powerful, broad-specificity approach is ideal for gene expression from a very small number of cells, such as exosomes or single cells, without the need for harsh chemicals or further purification.
- Ideal for preparing RNA from mammalian cell culture, laser capture microdissection, FACS-prepared cell populations, cells in suspension, adherent cells, cells stored in RNAlaterTM and cell pellets
- Simplified, hands-off workflow provides RT-PCR and RT-qPCR ready RNA in approximately 5 minutes
- Releases both RNA and DNA with excellent recovery across a wide range of cell numbers
- Requires no further purification for accurate RT-PCR and RT-qPCR analysis
- Inhibitor-free means no harsh chemical washes or multiple steps
- RT-PCR and RT-qPCR reagents can be added directly to the lysate for a simple, streamlined protocol in 96 well plates
- Each kit contains RNAGEM, DNase I, BLUE buffer, 10x DNase buffer, and 10x TE
Learn more about RNAGEM and recent applications. Download our RNAGEM technical overview. Watch our webinar on advances in extracting and stabilizing RNA.
prepGEM Universal
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
- Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
- Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
- Perfect for CRISPR/Cas9 genotyping workflows
- Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer
Latest Resources
DNA extraction: Proteinase K versus prepGEM
Efficient DNA extractions for CRISPR toolkits: A simplified CRISPR genotyping workflow
Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice
Fast and simple DNA preparation for the screening of CRISPR/Cas9-generated knockout cell lines
Rapid DNA extraction from human saliva and blood for SNP calling