This webinar introduces a simple protocol for extracting DNA from insects using a single tube, without ionic detergents, magnetic beads, columns or centrifugation. It uses case studies featuring a range of insects including fruit flies, anopheles mosquitoes, and African whitefly, and presents qPCR data comparing enzymatic lysis with traditional spin-column methods.

Learning outcomes:

  • The differences between chemical and enzymatic, heat-driven DNA extractions
  • A method to extract DNA from small/scarce/challenging insect samples in less than 20 minutes
  • Optimizing extractions for downstream applications including PCR, qPCR, whole genome amplification, and amplicon sequencing

This webinar was recorded live by MicroGEM on April 1, 2020.

Related Products

prepGEM Universal

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

  • Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
  • Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
  • Perfect for CRISPR/Cas9 gene editing with NGS
  • Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

Learn more about prepGEM Universal for CRISPR applications. Download our technical overview, Efficient DNA Extractions for CRISPR toolkits: A Simplified CRISPR Genotyping Workflow.

View Product