This 15-minute webinar provides an overview of MicroGEM’s temperature-driven approach to DNA and RNA extractions where speed, sample size, yield, and simplified workflow are important. Single-tube extractions produce high-quality nucleic acids in minutes, not hours, without the overuse of plastic or harsh chemicals. Reduced handling means fewer opportunities for contamination, all at an affordable cost.

Gene therapy, molecular biology, pharmacology, forensics, environmental sciences, and plant pathology are just a few fields where MicroGEM is helping scientists move quickly to analysis, produce faster results, and make timely decisions about the implications of their studies.

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RNAGEM

Temperature-driven, single-tube extractions of total RNA from mammalian cells, tissues, insects, bacteria and virus

This powerful, broad-specificity approach is ideal for gene expression from a very small number of cells, such as exosomes or single cells, without the need for harsh chemicals or further purification.

  • Ideal for preparing RNA from mammalian cell culture, laser capture microdissection, FACS-prepared cell populations, cells in suspension, adherent cells, cells stored in RNAlaterTM and cell pellets
  • Simplified, hands-off workflow provides RT-PCR and RT-qPCR ready RNA in approximately 5 minutes
  • Releases both RNA and DNA with excellent recovery across a wide range of cell numbers
  • Requires no further purification for accurate RT-PCR and RT-qPCR analysis
  • Inhibitor-free means no harsh chemical washes or multiple steps
  • RT-PCR and RT-qPCR reagents can be added directly to the lysate for a simple, streamlined protocol in 96 well plates
  • Each kit contains RNAGEM, DNase I, BLUE buffer, 10x DNase buffer, and 10x TE

Learn more about RNAGEM and recent applications. Download our RNAGEM technical overview. Watch our webinar on advances in extracting and stabilizing RNA.

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prepGEM Universal

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

  • Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
  • Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
  • Perfect for CRISPR/Cas9 gene editing with NGS
  • Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

Learn more about prepGEM Universal for CRISPR applications. Download our technical overview, Efficient DNA Extractions for CRISPR toolkits: A Simplified CRISPR Genotyping Workflow.

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prepGEM Bacteria

Suitable for extracting DNA from bacteria (Gram-positive and Gram-negative), protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water

Produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, 16S rDNA sequencing

Each kit contains prepGEM, lysozyme, GREEN+ buffer, enhancer and wash

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PDQeX phytoGEM

Suitable for studying plant genotypes and genetic variation, endophytic plant pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and for evaluating the authenticity of exported plant varieties, botanical extracts and molecular detection of food adulterant.

Produces double-stranded DNA suitable for PCR and qPCR.

Each kit contains PDQeX prepGEM, Histosolv, enhancer, GREEN+ buffer, phytocards, and extractor tubes.

 

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PDQeX Nucleic Acid Extractor

MicroGEM’s automated solution for effortless DNA extraction.

Ideal for PCR, qPCR, STR, and Whole Genome Sequencing.

Optimized for lysis and purification for double-stranded sequencing; also produces high-quality single-stranded extractions.

Simplified workflow in a single closed-tube system to handle challenging samples such as plant and fungi.

Perfect for labs looking for fast, hands-free, programmable sample processing.

Produces DNA in less than 20 minutes

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forensicGEM Sperm

Extracts DNA from sperm cells, semen samples, and vaginal swabs

Produces single-stranded DNA validated for use with STR, PCR, and qPCR.

Each extraction kit contains forensicGEM Sperm, Acrosolv, and ORANGE+ buffer

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forensicGEM Universal

MicroGEM's forensicGEM Universal Kit provides users with a single tube solution for extracting DNA from a variety of forensically-relevant human samples.

Extracts DNA from forensic samples including saliva (liquid, swab, stains, storage card – FTA), blood (liquid, swab, stains, storage card – FTA), and tissue (solid, hair)

Produces high-quality single-stranded DNA proven for use with STR, PCR, qPCR and whole genome amplification

Each kit contains forensicGEM, Histosolv, BLUE buffer, RED+ buffer and ORANGE+ buffer

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