These validation studies confirm the efficacy of forensicGEM sperm to rapidly prepare samples for Y screening. Lysis of sperm and epithelial cells in mixtures can be quantified using real time PCR to detect male DNA. Further, this process provides a good estimate of male DNA to total genomic DNA. Finally, the real time PCR data from the soiled denim was useful in estimating DNA concentration for STR amplification, indicating that the extraction can handle common inhibitors such as soil and denim.

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