Isolating nucleic acids is often the rate-limiting step in molecular diagnostics. Removing inhibitory substances and preparing high-quality extracts with a quick turn-around time are critical for successful downstream processing.
MicroGEM’s PDQeX Nucleic Acid Extractor offers a simple, nearly effortless solution for DNA and RNA extraction for:
Whole Genome Sequencing
MicroGEM’s PDQeX Nucleic Acid Extractor brings together powerful proteinases and mesophilic cell wall degrading enzymes with an innovative extractor cartridge, creating a closed-tube system to lyse cells, destroy nucleases, digest proteins, and release nucleic acids.
This approach prevents contamination and has no laborious sample preparation, while producing extracts in significantly less time (minutes rather than hours) than other extraction methods.
Interested in seeing the PDQeX Nucleic Acid Extractor in action? View a quick demonstration to see how the PDQeX provides labs with fast, hands-free sample preparation. Just load the instrument, select a protocol, and press ‘Start' for high-quality DNA and RNA in minutes.
The PDQeX is also the ideal solution for processing tough samples such as plant stems and roots. Thermophilic proteinase and mesophilic cell wall degrading glycosyl hydrolases, used with MicroGEM's innovative extraction cartridge, provide plant to PCR-ready DNA in less than 15 minutes. See a demonstration of MicroGEM's phytoGEM system, including crushing plant material onto a storage card and extracting high-quality DNA for genotyping, sequencing, and the detection of pathogens.
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Download a comprehensive product guide here.
Download the current PDQeX product manual here.
Latest PDQeX news from our researchers and collaborators
Tree Lab: Portable genomics for early detecting of plant viruses and pests in Sub-Saharan Africa. Genes. 10(9), 2019 (pdf).
This study demonstrates a technical breakthrough in the ability to conduct bacterial DNA extraction and genomic analysis in the field. MicroGEM's PDQeX Nucleic Acid Extractor, along with a collaborator's portable sequencing technologies, performed real-time identification of viral pathogens in a farmer's field setting.
Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX. BioTechniques. 66(5), 2019 (pdf).
This study compared a conventional column purification method with the PDQeX Nucleic Acid Extractor for purifying DNA for whole genome sequencing. Based on six bacterial suspensions, the PDQeX performed much more rapidly – in under 30 minutes – while producing comparable high-quality extractions.
Shaffer, L., Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences. 116(9), 2019 (pdf)
The article describes the ability to screen Cassava crops for Cassava Mosaic Virus (CMV) using MicroGEM's PDQeX for rapid DNA extraction. The PDQeX was used as the enabling technology to complement DNA sequencing being conducted in the field in real-time.