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The PDQeX forensicGEM Universal kit offers a simple hands-off method for DNA extraction and purification of a wide variety of forensic samples including blood, saliva and tissue.
Using MicroGEM’s PDQeX Nucleic Acid Extractor, there are only two simple steps – quick sample prep and temperature-controlled DNA extraction and purification. This closed-tube, automated approach generates purified double-stranded DNA ready for high-throughput screening and works perfectly for STR, PCR and qPCR applications.
DNA extraction from bone is challenging. Complex sampling and purification steps are time-consuming and may lead to contamination. When processed using MicroGEM’s PDQeX Nucleic Acid Extractor and the PDQeX forensicGEM Universal kit, DNA from bone can be extracted much quicker than standard bone preparation methods. The single step, closed tube temperature-controlled method reduces handling of samples, lowers the risk of contamination, and decreases extraction time.
The Forensic Science Laboratory of the French Gendarmerie compared MicroGEM’s temperature-controlled PDQeX approach to regular bone preparation using a rotary saw and grinder. Using a fragment of bone from a burned body, the study demonstrated the PDQeX approach is much quicker, generating full DNA profiles in less than 2.5 hours compared to 6 hours using the other approach. The relative fluorescence intensity (rfu) was 3 times higher using PDQeX than the standard protocol.
TREPAN and GO: hDNA Collecting and Profiling from Bones is not Challenging Anymore (pdf)
High throughput laboratories for paternity, forensics, and genetic testing frequently process thousands of buccal per year. Most sample preparation methods are difficult to automate and, when automated, consume vast amounts of pipette tips and other consumables not included in the extraction kits. The extraction chemistries often require very specialized robotics and consumables.
forensicGEM Universal is ideal for high throughput processing of buccal swabs on a laboratory’s existing thermal cycler, requiring only a small cutting from the sample. Single-step, closed tube processing enables high automation, rapid lysis, and efficient extraction. This approach has been proven in labs processing over 10,000 samples per year.