Join Dr. Anastasia Malek as she shares her experience isolating specific populations of the thyroid- and the prostate-derived ECnV followed by miRNA isolation and analysis.

Small extracellular nano-vesicles (SEnV) circulating with plasma is a heterogeneous population of vesicles secreted by different cells, mostly by blood cells and endothelium. A small fraction of this population is derived from other cells and tissues in amounts apparently proportional to the tissue mass and secretory activity. This small fraction has a great diagnostic potency since molecular composition of these vesicles may reflect the condition of specific tissue or cell type. Therefore, isolation and analysis of specific disease-indicative SEnV present exciting opportunities to develop new approaches for cancer diagnostic and management.

The pattern of cellular miRNAs is affected during malignant transformation. Cancer-associated shift of miRNA profile of malignant cells can be translated into ECnVs secreted by these cells and available for analysis from the plasma sample. This is a main reason why miRNA from tissue-specific population of plasma SEnV is considered to be a promising cancer marker. However, isolation of miRNA from small vesicular population is an extremely tricky process vulnerable to loss of material. RNAGEM isolates the miRNA from small samples, to reduce loss of material, and to minimize alteration of miRNA profile.

This 20-minute talk is followed by a Q&A session co-hosted by Giuseppina Sannino, PhD, MicroGEM’s Regional Innovation and Business Manager.

Learn about:
– A new approach to isolate total population of plasma SEnV
– Options to isolate tissue-specific population of plasma SEnV
– Isolation of vesicular miRNA with RNAGEM

Anastasia Malek, MD, PhD
Senior Scientist, Head of Sub-cellular Technology Lab (SCT-Lab)
N.N.Petrov National Medical Research Center of Oncology, Saint-Petersburg
Anastasia Malek studies extracellular nanovesicles for cancer diagnostics and therapy. She is also Co-founder and Director at Oncosystem Ltd focusing on vesicular and tissue miRNA analysis for cancer diagnostics. Anastasia earned a PhD in Oncology/Biochemistry from the Petrov Institute of Oncology and earned her medical degree in General Medicine from St. Petersburg State Medical University. She completed postgraduate work in Surgery/Oncology at St. Petersburg Medical Academy of Postgraduate Studies and in Polymer-based Transfection Technologies at Philipps-University School of Medicine.

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