Discover how a simple, single-tube protocol can help you efficiently process your sexual assault samples, including Y screening and differential extractions.
Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification.
There is a simpler way.
Jeff shows qPCR and STR data comparing the enzymatic chemistry to conventional sperm lysis methods. He also discusses its use in Y screening of sexual assault evidence, as well as its use in differential extraction protocols.