In this application note, researchers at the Technical University Munich describe prepGEM Universal as a rapid and simple method to extract DNA suitable for PCR and Sanger Sequencing analysis.
prepGEM Universal was used for the extraction of DNA from single cell colonies after CRISPR-mediated editing to perform screening of the clones. The extraction was performed 10 days after the transfection using a 96-well plate. The data here reported shows that prepGEM Universal is well suited for CRISPR screening.
Read the app note here.