Traditional DNA and RNA extraction methods often rely on proteinase K, SDS, and purification by columns, beads, or solvent extraction. Extraneous agents are added that subsequently need to be removed – all in an effort to get a simple result – high-yield, purified nucleic acids.
Much of the problem is the inadequacy of the lysis step. Proteinase K doesn’t work at a high enough temperature to denature the proteins it is digesting. To compensate, SDS is added but is toxic to every downstream diagnostic. So it must be removed, initiating a cascade of steps – each solving what was done in the previous step.
Time is wasted.
Yields aren’t preserved.
Errors are made.
Samples are vulnerable to contamination.
Technicians are exposed to harsh chemicals.
Excess plastic waste and hazardous materials need to be thrown away.
The MicroGEM Advantage
At MicroGEM, we have re-invented DNA and RNA extraction. We replace traditional extraction methods with a simple, single-tube approach. Our thermophilic and mesophilic enzymes are derived from extremophiles living in the world’s harshest environments. Our specially-formulated cocktails of these enzymes and buffers work at different temperatures, creating a sequence of coordinated temperature-driven reactions easily done using a thermal cycler or MicroGEM’s PDQeX Nucleic Acid Extractor.
Either way, our simplified sample preparation produces high-quality DNA and RNA, with good yields, less chance of error and less chance of cross-contamination.
By tapping into nature’s most extreme organisms and deriving enzymes from these extremophiles, we capture nature’s power in a single tube. As a result, we offer a nearly effortless approach to extracting high-quality nucleic acid from a single cell or from thousands of cells – all in minutes, not hours – with no loss of DNA or RNA, and at a reasonable cost.
Watch MicroGEM’s webinar featuring our single-tube approach to effortless nucleic acid extraction and discover how to simplify your workflow.
Workflow Comparison Using Temperature-Controlled Extraction vs Conventional Extraction Methods
The thermophilic proteinase activates with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment – a standard thermocycler can be programmed to carry out the extraction or use MicroGEM’s PDQeX Nucleic Acid Extractor for simple automation.
The process is a game-changer for challenging bacterial, fungal, and plant samples. Importantly, the low activity of the proteinase at mesophilic temperatures means enzymes such as lysozyme, glucuronidases, cellulases and hemicellulases can be activated to break down tough cell walls. Destroyed at 75°C, they clear the way for the proteinase to do its work.
The result? Single-tube nucleic acid extractions, even from challenging samples, compatible with most downstream applications.