MicroGEM’s PDQeX Nucleic Acid Extractor is the next generation of thermocyclers. It uses powerful enzyme-driven extraction reagents with an innovative extractor cartridge and purification matrix. Just add heat and the closed-tube system produces DNA extracts in significantly less time (minutes rather than hours) than other extraction methods.
PDQeX Quick-Start Guide prepGEM Bacteria (pdf)
PDQeX Quick-Start Guide forensicGEM Sperm (pdf)
Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX. BioTechniques. Vol.66, No. 5. 2019 (pdf)
This study compared a conventional column purification method with the PDQeX Nucleic Acid Extractor for purifying DNA for whole genome sequencing. Based on six bacterial suspensions, the PDQeX performed much more rapidly – in under 30 minutes – while producing comparable high-quality extractions.
Shaffer, L. Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences,116(9), 3351-3353. doi:10.1073/pnas. 1901806116. 2019 (pdf)
The article describes the ability to screen Cassava crops for Cassava Mosaic Virus (CMV) using MicroGEM’s PDQeX for rapid DNA extraction. The PDQeX was used as the enabling technology to complement DNA sequencing being conducted in the field in real-time.
MicroGEM’s PDQeX Nucleic Acid Extractor brings together powerful proteinases and mesophilic cell wall degrading enzymes with an innovative extractor cartridge, creating a closed-tube system to lyse cells, destroy nucleases, digest proteins, and release nucleic acids.
This approach prevents contamination and has no laborious sample preparation, while producing extracts in significantly less time (minutes rather than hours) than other extraction methods.