The PDQeX phytoGEM kit offers a simple hands-off method for DNA extraction and purification of a variety of plant samples.
Using MicroGEM’s PDQeX Nucleic Acid Extractor, there are only two simple steps – quick sample prep and closed tube automated DNA extraction and purification. The method provides quicker turn-around time for all plant sample types, even your toughest challenges.
Extracting DNA from plants can be challenging because of their tough cell walls and the inhibitory polysaccharides and tannins released during the extraction process. Tissue grinding followed by silica beads, columns or organic solvents becomes a multistep process, both labor-intensive and time-consuming.
The PDQeX phytoGEM kit provides a simple hands-off approach to produce high-quality DNA extraction for most plant-based molecular studies.
Sample prep is easy:
The PDQeX closed tube system uses our thermophilic proteinase combined with a cocktail of mesophilic cell wall degrading glycosyl hydrolases. These enzymes systematically lyse cells, destroy nucleases, digest proteins, and release DNA. Purification is achieved in the same closed tube system. The entire process, achieved on the PDQeX Nucleic Acid Extractor, achieves PCR-ready DNA in under 15 minutes, helping you to quickly find answers to urgent questions about your plant and your plant’s health.
Application Note: Rapid automated extraction of high-quality plant DNA using phytoGEM and the PDQeX Nucleic Acid Extractor (pdf)
Plant diseases cause an estimated 10-20% loss in agricultural productivity worldwide. Many pathogens go untreated resulting in unintended crop destruction due to disease or controlled crop destruction to contain spread. Early detection of pathogens is vital for successful crop management. It allows proactive control measures to be applied well before the establishment and spread of a disease.
Historically, a bottleneck to early detection and pathogen characterization is the DNA extraction process. Current extraction practices are time consuming, often requiring elaborate sample preparation followed by a laborious chemical lysis and purification process.
MicroGEM’s phytoGEM kit provides a simple hands-off approach to DNA extraction ideal for studying endophytic plant pathogens. Using MicroGEM’s proprietary extractor cartridge, precise temperature-controlled enzymatic lysis is performed on the automated PDQeX Nucleic Acid Extractor, helping you quickly find answers to urgent questions about your plant’s health.
phytoGEM has been validated for the detection of the following pathogens:
Shaffer, L. (2019). Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences,116(9), 3351-3353. doi:10.1073/pnas.1901806116. (pdf)
The article describes the ability to screen Cassava crops for Cassava Mosaic Virus (CMV) using MicroGEM's PDQeX for rapid DNA extraction. The PDQeX was used as the enabling technology to complement DNA sequencing being conducted in the field in real-time.
The introduction of molecular techniques has allowed significant steps to be made in genomics, and in many areas of applied mycology, molecular based approaches have become routine for strain characterization, pathogen detection and identification. Molecular methods are now giving major insights into how fungi function in the environment, where they are present, and how they interact with associated organisms.
DNA extraction from fungi comes with some of the same challenges faced with plants- tough cell walls and inhibitory polyphenols and polysaccharides. Additionally, many fungi are pathogenic and need to be handled carefully.
Conventional DNA extraction methods from fungal cells are very time-consuming or show poor recovery of DNA. PhytoGEM’s enzymatic lysis method effectively extracts DNA from fungal cells using a closed tube system to ensure precise temperature-controlled enzymatic lysis with no harsh chemical washes required. Purification is achieved in the same closed tube system protecting yields while reducing opportunities for contamination.
The PDQeX Nucleic Acid Extractor is the ideal solution for processing tough samples such as plant stems and roots. Thermophilic proteinase and mesophilic cell wall degrading glycosyl hydrolases, used with MicroGEM’s innovative extraction cartridge, provide plant to PCR-ready DNA in less than 15 minutes. MicroGEM’s phytoGEM approach to plant DNA extraction includes a convenient solution for crushing plant material onto a storage card. It is the ideal solution for extracting high-quality DNA for genotyping, sequencing, and the detection of pathogens.