This application note reports on the use of prepGEM as a DNA extraction method applied to multiple storage methods, including Queen’s lysis buffer, FTA cards, ethanol, and frozen whole blood. DNA from avian blood samples was extracted from the four different storage methods. The DNA yield for each storage type was significantly higher than with mammalian blood (this is expected given that avian blood cells are nucleated; as a result, very small amounts of avian blood may be used for DNA analysis). Sequencing from the barcoding products resulted in high-quality sequences. This extraction method for avian blood is rapid, reproducible, and provides DNA that can be reliably used for sexing and generating high quality DNA sequences.
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MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
Perfect for CRISPR/Cas9 gene editing with NGS
Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer