This paper describes DNA preparation using prepGEM, Whatman FTA cards, and a downstream post-PCR cleanup kit for Sequenom MALDI-TOF MS genotyping. Sequenom’s iPLEX Gold and MassARRAY matrix-assisted laser desorption / ionization time of flight (MALDI-TOF) mass spectrometry (MS) technology allows for high throughput, accurate and real-time scoring of up to 40 SNPs from DNA (or cDNA) of an individual. Single nucleotide polymorphisms (SNPs) are generally accepted as important markers owing to their abundance and simplicity; thus rendering straightforward assay design and scoring. Since a large number of samples is required to generate significant data, reagent costs can become prohibitory for research. Thus, a low cost and efficient DNA extraction method is required that produces good quality DNA template for downstream analysis. The MicroGEM prepGEM extraction kit provides a simple, less error-prone, low cost and rapid DNA extraction method.
Download Now will open in new tab