App Note
Efficient DNA Extractions for CRISPR Toolkits
prepGEM offers a simple, rapid DNA extraction solution that leverages a thermostable proteinase and single-tube protocol. The approach is easily scaled to high-throughput levels by simply using a 96-well plate and PCR machine. This allows researchers to genotype 96 cell lines simultaneously. With protocols as short as 4 minutes start to finish, a laborious process now becomes quick and very efficient.
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prepGEM Universal
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
- Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
- Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
- Perfect for CRISPR/Cas9 genotyping workflows
- Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer
Latest Resources
DNA extraction: Proteinase K versus prepGEM
Efficient DNA extractions for CRISPR toolkits: A simplified CRISPR genotyping workflow
Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice
Fast and simple DNA preparation for the screening of CRISPR/Cas9-generated knockout cell lines
Rapid DNA extraction from human saliva and blood for SNP calling