This webinar introduces a simple protocol for extracting DNA from insects using a single tube, without ionic detergents, magnetic beads, columns or centrifugation. It uses case studies featuring a range of insects including fruit flies, anopheles mosquitoes, and African whitefly, and presents qPCR data comparing enzymatic lysis with traditional spin-column methods.
This webinar was recorded live by MicroGEM on April 1, 2020.
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
Perfect for CRISPR/Cas9 gene editing with NGS
Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer