Video

On-Demand Webinar: Toward a Sustainable Next Generation Lab – Efficient Nucleic Acid Extraction to Improve Genome Engineering Workflow

Join Kalpana Surendranath, PhD, Senior Lecturer and Leader of the Genome Engineering Lab at the University of Westminster, to discover the next generation of sample preparation. While innovations in CRISPR-Cas have the potential to benefit everyone and help save lives, there is considerable demand to reduce resources required for the workflow, including reducing the time and expense to extract and purify nucleic acids. Dr. Surendranath and her team have successfully applied MicroGEM’s enzymatic extraction methods to establish a robust genome editing screen for cultured cell lines which reduced the workload drastically.

This 30-minute talk is followed by a Q&A session co-hosted by Giuseppina Sannino, PhD, MicroGEM’s Regional Innovation and Business Manager EMEA.

Learn about:
– a basic workflow of human genome engineering using the rapidly evolving CRISPR/Cas9 methodology
– sustainable ways of innovating human genome engineering workflow to support laboratory best practices
– an enzymatic approach to nucleic acid extraction that is simple and fast, with optimal recovery and easy automation advantages.

Meet the Speaker

Kalpana Surendranath PhD, PGCHE, FHEA, MRSB
Senior Lecturer, Leader of Genome Engineering Lab @University of Westminster, London UK
Dr. Kalpana Surendranath is a molecular cell biologist with expertise in CRISPR human genome editing, genome instability, and human diseases and protein engineering. As a faculty member at the University of Westminster, she undertakes both research and teaching on CRISPR genome engineering and runs the Institute of Biomedical Sciences (IBMS) accredited 3-day short course in human genome engineering. She received the Vice-Chancellor’s WestminSTAR award, the highest honour of the University, and is an Aurora AdvanceHE UK women leadership in higher education role model. She is a member of Genetics Society, Royal Society of Biology, British Society for Cell and Gene Therapy and Pharmacogenetics. She completed a PhD in Life Sciences at the Indian Institute of Science and worked as a postdoctoral fellow at the Institute of Molecular Cancer Research (IMCR), University of Zurich where she made significant contributions to reconstitution of human mismatch repair proteins in vitro.

 

 

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prepGEM Universal

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

  • Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
  • Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
  • Perfect for CRISPR/Cas9 genotyping workflows
  • Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

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