A comprehensive guide to using PDQeX for DNA extraction from: saliva, solid tissue, insects, mouse tails/ear cuts, blood, cultured cells, bacteria, soil, stool, plant leaves, stems, roots, plant pathogens, homogenized leaves or ground plant tissue, and fungal cultures.

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PDQeX Nucleic Acid Extractor

MicroGEM’s automated solution for effortless DNA extraction.

Ideal for PCR, qPCR, STR, and Whole Genome Sequencing.

Optimized for lysis and purification for double-stranded sequencing; also produces high-quality single-stranded extractions.

Simplified workflow in a single closed-tube system to handle challenging samples such as plant and fungi.

Perfect for labs looking for fast, hands-free, programmable sample processing.

Produces DNA in less than 20 minutes.

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PDQeX phytoGEM

Suitable for studying plant genotypes and genetic variation, endophytic plant pathogens, genetic mapping, fingerprinting, microsatellite marker-assisted selection, and for evaluating the authenticity of exported plant varieties, botanical extracts and molecular detection of food adulterant.

Produces double-stranded DNA suitable for PCR and qPCR.

Each kit contains PDQeX prepGEM, Histosolv, enhancer, GREEN+ buffer, and extractor tubes.

For processing larger samples, we recommend the PDQeX phytoGEM HC kits. These kits have the traditional advantages that come with using the PDQeX Nucleic Acid Extractor but also include the new HC extraction cartridges.

These new HC extraction cartridges allow for more sample to be loaded into the cartridge before the purification matrix becomes overloaded. This results in a higher final yield of extracted DNA, meaning the PDQeX phytoGEM HC kits are better suited for sequencing workflows where a higher yield of DNA is encouraged.

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PDQeX phytoGEM Punch Tool and Tip

Used with MicroGEM storage cards to cut out plant sample and transfer to a PDQeX cartridge.

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PDQeX phytoGEM Punch Tool Replacement Tip

Used with MicroGEM storage cards to cut out plant sample and transfer to a PDQeX cartridge.

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PDQeX prepGEM Bacteria

Suitable for extracting DNA from Gram-positive bacteria, Gram-negative bacteria, protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool, and water.

Double-stranded DNA extracts suitable for PCR, qPCR, targeted amplicon sequencing from metagenomic samples and whole genome sequencing.

Each kit contains PDQeX prepGEM, lysozyme, enhancer, GREEN+ buffer, wash, and extractor tubes.

For processing larger samples, we recommend the PDQeX prepGEM Bacteria HC kits. These kits have the traditional advantages that come with using the PDQeX Nucleic Acid Extractor but also include the new HC extraction cartridges.

These new HC extraction cartridges allow for more sample to be loaded into the cartridge before the purification matrix becomes overloaded. This results in a higher final yield of extracted DNA, meaning the PDQeX prepGEM Bacteria HC kits are better suited for sequencing workflows where a higher yield of DNA is encouraged.

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PDQeX prepGEM Universal

Effortlessly extract DNA from saliva, blood, tissue including animal (ear punches, mouse tails, insect), and cell culture.

Produces double-stranded DNA for STR, PCR, qPCR, or amplicon sequencing.

Each kit contains PDQeX prepGEM, Histosolv, BLUE buffer, ORANGE+ buffer, enhancer, and extractor tubes.

For processing larger samples, we recommend the PDQeX prepGEM Universal HC kits. These kits have the traditional advantages that come with using the PDQeX Nucleic Acid Extractor but also include the new HC extraction cartridges.

These new HC extraction cartridges allow for more sample to be loaded into the cartridge before the purification matrix becomes overloaded. This results in a higher final yield of extracted DNA, meaning the PDQeX prepGEM Universal HC kits are better suited for sequencing workflows where a higher yield of DNA is encouraged.

 

Read our latest application notes:

Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice

Rapid DNA extraction from human saliva and blood for SNP calling

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phytoGEM Cards

Used with the PDQeX phytoGEM Crusher Tool and Punch Tool when transferring plant samples for processing and storage.

Available in 500 or 1000 units.

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phytoGEM Starter Kit

The phytoGEM Starter Kit is MicroGEM’s all-in-one solution for preparing and processing plant DNA samples.

Includes 1 crusher tool, 1 punch and tip, 50 phytocards, and 50 phytoGEM reactions.

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