App Note
Quantifying MicroGEM nucleic acid extracts
MicroGEM’s Temperature Controlled Extraction method (TCE) utilises the robust activity of MicroGEM’s thermophilic protease, prepGEM, to extract nucleic acids from a multitude of samples. MicroGEM’s TCE methodology does not introduce downstream inhibitors into the sample, such as
ionic detergents like SDS, but instead uses the combination of heat and protease activity to extract nucleic acids in a single-tube, by simply adjusting the temperature. As MicroGEM’s TCE does not require the use of harsh detergents to extract nucleic acids, and the extraction buffers are compatible with downstream analysis, extracted DNA/RNA generally does not need to be purified before
analysis can take place.
For this reason, MicroGEM is able to extract nucleic acids quickly and simply with an unparalleled DNA/RNA recovery and a reduced risk of contamination. In some cases the extracts need to be quantified to ensure the correct amount of DNA/RNA is used as a template for the next step.
In this application note, we look at some common methods for nucleic acid quantification (UVspectrophotometry, florescent-dye and qPCR) and show the most accurate methods when working with MicroGEM extracts.
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Related Products
prepGEM Universal
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
- Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
- Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
- Perfect for CRISPR/Cas9 genotyping workflows
- Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer
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prepGEM Bacteria
Suitable for extracting DNA from bacteria (Gram-positive and Gram-negative), protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water.
Produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, 16S rDNA sequencing.
Each kit contains prepGEM, lysozyme, GREEN+ buffer, enhancer and wash.