This application note provides an analysis of prepGEM as an efficient DNA extraction method for mice and rats, shown to produce high-quality DNA for hundreds of samples in under an hour. In this comparative study, prepGEM yielded readily-amplified DNA with no migration distortion or smearing. Yield from the prepGEM approach was significantly higher than the other two commercially available kits (100.0 ng/mg of tissue from 2 mm tail tips for prepGEM vs 6.43 ng/mg and 1.83 ng/mg of tissue from 5 mm tail tips. prepGEM’s high performance is attributed to the high-temperature proteinase that releases DNA without chemical denaturants that can carry over into downstream reactions and cause reduced performance.
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MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
Learn more about prepGEM Universal for CRISPR applications. Download our technical overview, Efficient DNA Extractions for CRISPR toolkits: A Simplified CRISPR Genotyping Workflow.