RNAGEM is a powerful, broad-specificity, thermophilic proteinase that aggressively destroys ribonucleases while stabilizing RNA. It is ideal for preparing RNA from mammalian cell culture, laser capture microdissection and FACS-prepared cell populations, as well as cells in suspension, adherent cells, cells stored in RNAlater™, and cell pellets.
RNAGEM uses a rapid, single-tube protocol that releases RNA with excellent linearity across a wide range of cell numbers, from a single or a few cells to thousands of cells. The method is automatable, closed-tube, and does not require further purification of the RNA for accurate RT-qPCR analysis.
The RNAGEM reagents efficiently lyse the cells, hydrolyse nucleases, and clear protein from strands of nucleic acids, preparing them for analysis. Greater sensitivity is achieved, especially with low abundance transcripts and small sample volumes as all nucleic acids are preserved in the process. Reduced handling and efficient template preparation mean RNAGEM generates mRNA profiles as close to the biological reality of the sample as possible.
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