In this application note, researchers at Vaxxinova Nederland collaborated with researchers at MicroGEM to compare the prepGEM Universal workflow with ‘traditional' proteinase K (PK) digestion.
Viral DNA was extracted from infected cells using the traditional PK protocol and the MicroGEM protocol. While both approaches produced satisfactory results, the MicroGEM approach produced stronger bands, especially starting from low abundant material. Overall, the MIcroGEM single-tube extraction and amplification required less hands-on time per reaction; less plastic and hazardous waste, less analysis time, lower costs for plastic consumables, and better turn-around time.
Read the app note here.