MicroGEM DNA extraction kits have simple, well-defined protocols that are outlined in our QuickStart Guides. All of the procedures have been optimised on a range of samples, but for unusual sample types, the procedures and formulations may need to be fine-tuned. This guide is provided to assist with troubleshooting and method optimisation. It highlights which steps are essential for success and which can be changed. It includes tips for improving your PCR, solving PCR problems, and workflow optimisation.
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Extracts DNA from forensic samples including saliva (liquid, swab, stains, storage card – FTA), blood (liquid, swab, stains, storage card – FTA), and tissue (solid, hair)
Produces high-quality single-stranded DNA proven for use with STR, PCR, qPCR and whole genome amplification
Each kit contains forensicGEM, Histosolv, BLUE buffer, RED+ buffer and ORANGE+ buffer
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
Perfect for CRISPR/Cas9 gene editing with NGS
Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer
Suitable for extracting DNA from bacteria (Gram-positive and Gram-negative), protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water
Produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, 16S rDNA sequencing
Each kit contains prepGEM, lysozyme, GREEN+ buffer, enhancer and wash