MicroGEM DNA extraction kits have simple, well-defined protocols that are outlined in our QuickStart Guides. All of the procedures have been optimised on a range of samples, but for unusual sample types, the procedures and formulations may need to be fine-tuned. This guide is provided to assist with troubleshooting and method optimisation. It highlights which steps are essential for success and which can be changed. It includes tips for improving your PCR, solving PCR problems, and workflow optimisation.

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forensicGEM Sperm

Extracts DNA from sperm cells, semen samples, and vaginal swabs

Produces single-stranded DNA validated for use with STR, PCR, and qPCR.

Each extraction kit contains forensicGEM Sperm, Acrosolv, and ORANGE+ buffer

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forensicGEM Universal

MicroGEM's forensicGEM Universal Kit provides users with a single tube solution for extracting DNA from a variety of forensically-relevant human samples.

Extracts DNA from forensic samples including saliva (liquid, swab, stains, storage card – FTA), blood (liquid, swab, stains, storage card – FTA), and tissue (solid, hair)

Produces high-quality single-stranded DNA proven for use with STR, PCR, qPCR and whole genome amplification

Each kit contains forensicGEM, Histosolv, BLUE buffer, RED+ buffer and ORANGE+ buffer

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prepGEM Universal

MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.

  • Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
  • Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
  • Perfect for CRISPR/Cas9 gene editing with NGS
  • Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer

Learn more about prepGEM Universal for CRISPR applications. Download our technical overview, Efficient DNA Extractions for CRISPR toolkits: A Simplified CRISPR Genotyping Workflow.

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prepGEM Bacteria

Suitable for extracting DNA from bacteria (Gram-positive and Gram-negative), protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water

Produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, 16S rDNA sequencing

Each kit contains prepGEM, lysozyme, GREEN+ buffer, enhancer and wash

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