MicroGEM DNA extraction kits have simple, well-defined protocols that are outlined in our QuickStart Guides. All of the procedures have been optimised on a range of samples, but for unusual sample types, the procedures and formulations may need to be fine-tuned. This guide is provided to assist with troubleshooting and method optimisation. It highlights which steps are essential for success and which can be changed. It includes tips for improving your PCR, solving PCR problems, and workflow optimisation.

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forensicGEM Sperm

Extracts DNA from sperm cells, semen samples, and vaginal swabs.

Produces single-stranded DNA validated for use with STR, PCR, and qPCR.

Each extraction kit contains forensicGEM Sperm, Acrosolv, and ORANGE+ buffer.

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forensicGEM Universal

MicroGEM's forensicGEM Universal Kit provides users with a single tube solution for extracting DNA from a variety of forensically-relevant human samples.

Extracts DNA from forensic samples including saliva (liquid, swab, stains, storage card – FTA), blood (liquid, swab, stains, storage card – FTA), and tissue (solid, hair).

Produces high-quality single-stranded DNA proven for use with STR, PCR, qPCR and whole genome amplification.

Each kit contains forensicGEM, Histosolv, BLUE buffer, RED+ buffer and ORANGE+ buffer.

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prepGEM Bacteria

Suitable for extracting DNA from bacteria (Gram-positive and Gram-negative), protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water.

Produces single-stranded DNA suitable for PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples, 16S rDNA sequencing.

Each kit contains prepGEM, lysozyme, GREEN+ buffer, enhancer and wash.

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