Jeff Chapman, MicroGEM’s CEO, discusses advances made in RNA extraction and stabilization, even from rare samples to a single cell, using a series of enzymes which efficiently lyse cells, clear interacting proteins from nucleic acids and eliminate RNases. Jeff discusses the technology behind this approach and provides examples from published literature.

Learn about:

  • An enzymatic approach to extracting and stabilizing RNA
  • Improving gene expression quantitation through more efficient recovery of RNA
  • Tackling gene expression from a few cells to a single cell
  • The future of democratized molecular devices for gene expression.

This webinar was presented live on Wednesday, May 13, 2020 at the Select Science Virtual Event on Cancer and Immunology Research.

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RNAGEM

Temperature-driven, single-tube extractions of total RNA from mammalian cells, tissues, insects, bacteria and virus

This powerful, broad-specificity approach is ideal for gene expression from a very small number of cells, such as exosomes or single cells, without the need for harsh chemicals or further purification.

  • Ideal for preparing RNA from mammalian cell culture, laser capture microdissection, FACS-prepared cell populations, cells in suspension, adherent cells, cells stored in RNAlaterTM and cell pellets
  • Simplified, hands-off workflow provides RT-PCR and RT-qPCR ready RNA in approximately 5 minutes
  • Releases both RNA and DNA with excellent recovery across a wide range of cell numbers
  • Requires no further purification for accurate RT-PCR and RT-qPCR analysis
  • Inhibitor-free means no harsh chemical washes or multiple steps
  • RT-PCR and RT-qPCR reagents can be added directly to the lysate for a simple, streamlined protocol in 96 well plates
  • Each kit contains RNAGEM, DNase I, BLUE buffer, 10x DNase buffer, and 10x TE

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