Product Document
prepGEM Universal Product Overview
This product overview details the many benefits of MicroGEM’s prepGEM Universal extraction kit. prepGEM Universal utilizes MicroGEM’s thermophilic proteinase for simple, rapid DNA extraction with unprecedented recovery. This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.
Key Advantages:
- Single-tube extraction
- Protocols ranging from 4 to 18 minutes
- High DNA Recovery – Minimal loss of DNA during extraction
- No magnetic beads – No spin-columns – No harsh chemicals
- Protocols scalable from 1 cell to millions of cells
- Easily automated on a standard laboratory thermocycler or robotic platform
- Minimal plasticware required – Reduced waste
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prepGEM Universal
MicroGEM’s prepGEM Universal is a widely-used, adaptable reagent kit suitable for DNA extractions from life science samples. Because the entire extraction is done in a single-tube with no washes or additional steps, there is no loss of DNA, enabling extractions from even a single cell.
- Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture
- Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing
- Perfect for CRISPR/Cas9 genotyping workflows
- Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer
Latest Resources
DNA extraction: Proteinase K versus prepGEM
Efficient DNA extractions for CRISPR toolkits: A simplified CRISPR genotyping workflow
Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice
Fast and simple DNA preparation for the screening of CRISPR/Cas9-generated knockout cell lines
Rapid DNA extraction from human saliva and blood for SNP calling
PDQeX prepGEM Universal
Effortlessly extract DNA from saliva, blood, tissue including animal (ear punches, mouse tails, insect), and cell culture.
Produces double-stranded DNA for STR, PCR, qPCR, or amplicon sequencing.
Each kit contains PDQeX prepGEM, Histosolv, BLUE buffer, ORANGE+ buffer, enhancer, and extractor tubes.
For processing larger samples, we recommend the PDQeX prepGEM Universal HC kits. These kits have the traditional advantages that come with using the PDQeX Nucleic Acid Extractor but also include the new HC extraction cartridges.
These new HC extraction cartridges allow for more sample to be loaded into the cartridge before the purification matrix becomes overloaded. This results in a higher final yield of extracted DNA, meaning the PDQeX prepGEM Universal HC kits are better suited for sequencing workflows where a higher yield of DNA is encouraged.
Read our latest application notes:
Rapid extraction of DNA from limited FFPE material derived from lung tumour of engineered mice
Rapid DNA extraction from human saliva and blood for SNP calling
PDQeX Nucleic Acid Extractor
MicroGEM’s automated solution for effortless DNA extraction.
Ideal for PCR, qPCR, STR, and Whole Genome Sequencing.
Optimized for lysis and purification for double-stranded sequencing; also produces high-quality single-stranded extractions.
Simplified workflow in a single closed-tube system to handle challenging samples such as plant and fungi.
Perfect for labs looking for fast, hands-free, programmable sample processing.
Produces DNA in less than 20 minutes.