On-Demand Forensic Webinar
Watch Now

Join Jeff Hickey, MicroGEM's forensic expert and product specialist, for this on-demand webinar to discover how our simple, single-tube protocol can help efficiently process sexual assault samples, including Y screening and differential extractions.

Reducing agents, such as DTT, have been used to extract DNA from sperm cells in forensic sexual assault samples for many years. These reducing agents require harsh detergents and denaturants in the lysis buffer to reduce disulfide bonds. The resulting lysate contains reagents and proteins that must be removed via purification steps prior to qPCR and/or STR amplification.

This on-demand webinar explains the benefits of temperature-driven, enzymatic sperm lysis including:

  • improved recovery of DNA
  • speed
  • simplified workflow and
  • significant reduction of plastic and chemical waste.

Jeff shows qPCR and STR data comparing the enzymatic chemistry to conventional sperm lysis methods. He also discusses its use in Y screening of sexual assault evidence, as well as its use in differential extraction protocols.

Read more about the comparative analysis here.

Find product information here.

This webinar was recorded live by MicroGEM on February 11, 2020.